根区增温对玉米幼苗主要代谢物傅里叶红外光谱特性及叶绿素含量的影响

doi:10.3964/j.issn.1000-0593(2020)04-1283-06

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摘要：为深入研究根区温度对玉米幼苗主要代谢物以及叶绿素含量的影响，试验设置常温（24 ℃）、中温（30 ℃）和高温（36 ℃）三个根区温度水平，以SD609（保绿型）和SD902（衰老型）两个不同抗逆型玉米品种为样品，采用傅里叶变换红外光谱仪（Tensor Ⅱ，德国布鲁克）测定玉米幼苗根、茎、叶三个器官的光谱特征。结果表明：不同根区温度处理对玉米幼苗各个器官有机物含量有显著影响，但影响程度不同，大体表现为根>茎>叶；玉米幼苗根、茎、叶在3 330，2 927，1 639，1 515，1 350，1 250和1 055 cm^-1等波数处透射率均受根区温度的影响，且在根区中温条件下各器官上述波数处透射率较低，根区高温条件下透射率较高，在1 055 cm^-1处各处理差异最为明显；不同抗逆型品种在根区增温条件下FTIR光谱特征有较大差异，抗逆性较差的衰老型品种（SD902）在不同根区温度处理下FTIR光谱特征差异较大，叶绿素含量受根区增温影响较大，而抗逆性较好的保绿型品种（SD609）不同处理间差异较小，叶绿素含量受根区增温影响较小；根区温度对不同玉米品种根系活力及叶绿素含量影响均表现为中温（30 ℃）>对照（24 ℃）>高温（36 ℃）。因此，适当提高根区温度可以显著提高玉米幼苗根系活力、叶绿素、碳水化合物尤其是多糖含量，以及茎秆和叶片中蛋白质、核酸含量，但温度过高会使多糖、脂类、叶绿素含量以及根系活力迅速下降；与SD609相比，SD902对根区温度反应较为敏感，在根区高温条件下，玉米内源物质合成受阻，而SD609能保持相对较高的多糖及叶绿素含量，对根区高温条件有相对较好的适应性。根区温度对玉米幼苗根系作用最大，可通过影响根系吸收、物质合成及物质转运功能，进而影响茎秆、叶片有机物以及叶绿素含量。

关键词：玉米；根区增温；傅里叶变换红外光谱；叶绿素; 根系活力

Abstract：In order to study the effects of root zone temperature on the main metabolites and chlorophyll content of maize seedlings, the spectral characteristics and chlorophyll content of two maize cultivars under three root zone temperature levels were determined by Fourier transform infrared spectroscopy and SPAD-502 chlorophyll meter. The results showed that different root zone temperature treatment had significant effects on the organic matter content of various organs of maize seedlings, but the degree of influence was different, and the general realization was root>stem>leaf. The transmission of wave number of maize seedlings in 3 330, 2 927, 1 639, 1 515, 1 350, 1 250 and 1 055 cm^-1 was affected by the temperature of the root zone. The peak value was lower under medium temperature conditions and higher under the high temperature condition of the root zone, and the difference was most obvious at 1 055 cm^-1. The FTIR infrared spectral characteristics of different varieties in the root zone temperature range are quite different, and SD902 has a large difference, while SD609 has a small difference. The temperature of root zone had significant effects on the root activity and chlorophyll content of different maize varieties, and both showed medium temperature (30 ℃)>control (24 ℃)>high temperature (36 ℃). In summary, appropriate increase of root zone temperature can significantly increase the content of chlorophyll, root activity, carbohydrates, especially polysaccharides in corn seedlings, as well as protein and nucleic acid content in stems and leaves, but excessive temperature will rapidly decrease the content of polysaccharides, root activity, lipids and chlorophyll. The increase in root zone temperature has a significant effect on the seedling metabolites of SD902 and SD609 corn varieties, and the SD902 is more sensitive to the temperature response in the root zone, and the SD609 has better heat resistance. The root zone temperature had the greatest effect on the roots of maize seedlings, and by effecting the roots absorption, material synthesis and material transport functions, and then affect the stalk, leaf organic matter and chlorophyll content.

Key words：Maize; Lower ground temperature; Fourier transform infrared spectroscopy; Chlorophyll; Root activity

收稿日期: 2019-09-17 修订日期: 2019-12-30

中图分类号:

S513

基金资助: 国家自然科学基金项目（31771724），陕西省科技重点研发计划项目（2018NY-031），西北农林科技大学推广专项（TGZX2018-10）资助

通讯作者: 路海东 E-mail: lhd2042@163.com

作者简介: 夏镇卿，1996年生，西北农林科技大学农学院硕士研究生 e-mail: 2716555460@qq.com

引用本文:

夏镇卿，司雷勇，金岩，扶亚芳，王奇，路海东. 根区增温对玉米幼苗主要代谢物傅里叶红外光谱特性及叶绿素含量的影响[J]. 光谱学与光谱分析, 2020, 40(04): 1283-1288.
XIA Zhen-qing, SI Lei-yong, JIN Yan, FU Ya-fang, WANG Qi, LU Hai-dong. Effects of Root Zone Temperature Increase on Fourier Transform Infrared Spectroscopy Content of Main Metabolites and Chlorophyll in Maize Seedlings. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1283-1288.

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https://www.gpxygpfx.com/CN/10.3964/j.issn.1000-0593(2020)04-1283-06 或 https://www.gpxygpfx.com/CN/Y2020/V40/I04/1283