Determination of Chinese Honey Adulterated with Syrups by Near Infrared Spectroscopy Combined with Chemometrics
HUANG Fu-rong1, SONG Han1, GUO Liu1, YANG Xin-hao1, LI Li-qun2, ZHAO Hong-xia2*, YANG Mao-xun3*
1. Opto-electronic Department of Jinan University，Guangzhou 510632，China
2. Guangdong Institute of Applied Biological Resources, Guangzhou 510636，China
3. Zhuhai Dahengqin Science and Technology Development Co., Ltd., Zhuhai 519000，China
Abstract：To find a fast, accurate, and effective method for the identification of honey adulteration, near-infrared spectroscopy combined with chemometrics was used to analyze natural honey and adulterated honey in this paper. First, 224 samples were collected for the study, including 112 natural pure honey samples from 20 common honeys in China, and 112 adulterated honey sample were prepared with 6 different syrup samples according to different syrup contents(10%, 20%, 30%, 40%, 50%, or 60%). Near infrared spectral data (wavelength range of 400～2 500 nm) of all samples were obtained by near infrared light instrument scanning. Then, first derivative (FD), second derivative (SD), multiple scattering correction (MSC), and standard normal variation (SNVT) pre-processing of the original spectra combined with PLS-DA (linear algorithm) and SVM (non-linear algorithm) modeling, respectively, were adopted to establish a differential model of natural honey and syrup-adulterated honey and compare the effects of different pretreatment methods on the honey adulteration identification model established by the two different modeling algorithms. The penalty parameter c and the kernel function parameter g of the SVM algorithm were optimized by three optimization algorithms: grid search, genetic algorithm, and particle swarm optimization. The analysis results showed that the PLS-DA model established by the FD preprocessing had the best effect, and the accuracy of the best PLS-DA model was 87.50%. After MSC pre-processing, the SVM model with the penalty parameter c of 3.031 4 and the kernel function parameter g of 0.329 8 was the best. The accuracy of the best SVM model was 94.64%. It can be seen that the non-linear SVM algorithm combined with the NIR spectral data natural honey and syrup-adulterated honey identification model is better than the PLS-DA model.
黄富荣，宋 晗，郭 鎏，杨心浩，李立群，赵红霞，杨懋勋. 近红外光谱结合化学计量学的常见中国蜂蜜掺杂糖浆鉴别[J]. 光谱学与光谱分析, 2019, 39(11): 3560-3565.
HUANG Fu-rong, SONG Han, GUO Liu, YANG Xin-hao, LI Li-qun, ZHAO Hong-xia, YANG Mao-xun. Determination of Chinese Honey Adulterated with Syrups by Near Infrared Spectroscopy Combined with Chemometrics. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(11): 3560-3565.
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