1. Beijing Center for Physical and Chemical Analysis, Beijing 100089, China 2. PLA Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China 3. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract：In the present paper, we applied Fourier transform infrared spectroscopy (FTIR) and second derivative infrared spectroscopy to establish a rapid and efficient analytical method for a preliminary quality control of medicinal herb extracts. The commonly used Chinese herb medicine, Rhizoma coptidis, was taken as an example. The main component-berberine in rhizoma coptidis was analyzed as target compound. The results indicated that the IR spectra of rhizoma coptidis herb, its water and alcohol extracts, exhibit their macroscopic fingerprint characters with fine repeatability, and the corresponding secondary derivative spectra further confirmed the spectral characteristics of the original IR spectra by enhancing spectral resolution. The typical and strongest absorption band of IR spectrum of berberine is at 1 505 cm-1,which is assigned to aromatic skeleton vibration band, and its intensities in spectra of rhizoma coptidis herb and its different extracts become the main identification standard of the contents of berberine in those samples. In addition, comparing the IR spectra of the medicinal herb and its extracts, it was shown that the main bioactive components, alkaloids in rhizoma coptidis herb, are enriched by extraction, and the contents of berberine in alcohol extracts of rhizoma coptidis are higher than those in water extracts. The spectroscopic technique described is a simple and rapid analytical technique, which could provide valuable information about chemical constituents of medicinal extracts for guiding further chromatographic analysis and separation improvement.
武彦文1,肖小河2,孙素琴3,刘红霞3 . 黄连不同提取物的红外光谱研究[J]. 光谱学与光谱分析, 2009, 29(01): 93-96.
WU Yan-wen1,XIAO Xiao-he2,SUN Su-qin3,LIU Hong-xia3. Study on Traditional Chinese Medicine Extracts of Rhizoma Coptidis by FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(01): 93-96.
[1] LI Chun-hua(李春华). World Science and Technology(Modernization of Traditional Chinese Medicine)(世界科学技术-中药现代化)，2001, 3(2): 34. [2] Zhou Q, Sun S Q, Zuo L. Vibrational Spectrosc., 2004, 36: 207. [3] PENG Yong, SUN Su-qin, ZHAO Zhong-zhen, et al(彭 勇, 孙素琴, 赵中振, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(6): 679. [4] Yu L, Sun S Q, Fan K F, et al. Spetrochim. Acta A: Mol. Biomol. Spectrosc., 2005, 62(1-3): 22. [5] Liu H X, Sun S Q, Lü G H, et al. Vibrational Spectrosc., 2006, 40: 202. [6] Liu H X, Sun S Q, Lü G H, et al. Spectrochim. Acta A: Mol. Biomol. Spectrosc., 2006, 64(2): 321. [7] The Pharmacopoeia Comittee of People’s Republic of China(国家药典委员会编). Chinese Pharmacopoeia Vol. Ⅰ(中国药典, 一部), 2005. 213. [8] Chuang W C, Young D Y, Liu KAO K L L, et al. J. Chromat., 1996, 755: 19. [9] Sun S W, Tseng H M. J. Pharm. Biomed. Anal., 2005, 37: 39. [10] LIAO Qing-wen, FAN Dong-li, XIAO Xiao-he(廖庆文，樊冬丽，肖小河). China Journal of Chinese Materia Medica(中国中药杂志), 2007, 32(3): 210. [11] YING Yi, HE Zhi-hong, ZHOU Shi-wen, et al(应 懿, 何志红, 周世文, 等). Acta Academiae Medicinae Militaris Tertiae(第三军医大学学报), 2007, 29(9): 843. [12] LIU Yu-hong, LIU Yu-guo(刘玉红, 刘玉国). Chinese Journal of Modern Appl. Pharmacy(中国现代应用药学), 2007, 24(2): 140.
