Characterization of Structure of Carboxymethylpachymaran by IR Spectrum and Atomic Force Microscopy
WANG Bo1,SUN Run-guang1*,ZHANG Jing2
1. College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China 2. Department of Food Engineering, Shaanxi Normal University, Xi’an 710062, China
Abstract：The structure of Carboxymethylpachymaran was characterized using Fourier transform infrared spectroscopy. Morphological changes of carboxymethylpachymaran (CMP) were observed under different solution conditions by atomic force microscopy(AFM). After the carboxymethylation, the solubility of pachyman in the water was significantly increased, its β-D-glucan characteristic absorption peak at 890 cm-1 became weak obviously, and its methylene vibration absorption peak and CO antisymmetrical stretch vibration absorption peak appeared at 1 333 and 1 606 cm-1 respectively, which indicated that the carboxymethylation succeeded. The analysis of AFM results showed that CMP molecules existed in different morphology under different solution conditions, and that the concentration, ionic strength and solvent physical chemistry characteristics of polysaccharide solution had effects on the CMP chains conformation and the action mode between different molecular chains. The phenomena were considered to be related to hydrogen bond association and intramolecular and intermolecular electrostatic interactions of CMP. Meanwhile, the affinity and electrostatic interaction between CMP molecules and the mica substrate also had an influence on the CMP chains conformation and the image quality.
Key words：Atomic force microscopy;Infrared spectrum;Carboxymethylpachymaran
王博1,孙润广1*,张静2 . 羧甲基茯苓多糖结构的红外光谱表征与原子力显微镜观测[J]. 光谱学与光谱分析, 2009, 29(01): 88-92.
WANG Bo1,SUN Run-guang1*,ZHANG Jing2. Characterization of Structure of Carboxymethylpachymaran by IR Spectrum and Atomic Force Microscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(01): 88-92.
[1] Binning G, Quate C F, Gerber C. Phys. Rev. Lett., 1986, 56(9): 930. [2] Lyubchenko Y L, Shlyakhtenko L S. Proc. Natl. Acad. Sci. USA, 1997, 94(2): 496. [3] Hansma H G, Laney D E, Bezanilla M, et al. Biophys. J., 1995, 68(5): 1672. [4] Kumaki J, Hashimoto T. J. Am. Chem. Soc., 2003, 125(16): 4907. [5] Gunning A P, Kirby A R, Morris V J. Ultramicroscopy, 1996, 63(1): 1. [6] Mcintire T M, Penner R M, Brant D A. Macromolecules, 1995, 28: 6375. [7] Adamcik J, Klinov D V, Witz G, et al. FEBS Lett., 2006, 580(24): 5671. [8] Lee K Y, You H J, Jeong H G, et al. Int. Immunopharmacol., 2004, 4(8): 1029. [9] Hisanori K, Norihiko A, Masatoshi T. Chem. Pharm. Bull., 1983, 31(3): 1115. [10] Hamuro J, Yamashita Y, Ohsaka Y, et al. Nature, 1971, 233(5320): 486. [11] Burova T V, Golubeva I A, Grinberg N V, et al. Biopolymers, 1996, 39(4): 517. [12] Cesaro A, Gamini A, Navarini L. Polymer, 1992, 33(19): 4001. [13] Kaneda I, Kobayashi A, Miyazaw K, et al. Polymer, 2002, 43(4): 1301. [14] Boutebba A, Milas M, Rinaudo M. Biopolymers, 1997, 42(7): 811. [15] Boutebba A, Milas M, Rinaudo M. Int. J. Biological Macromol., 1999, 24(4): 319. [16] Wang Y F, Zhang L N, Ruan D. Chinese J. Polym. Sci., 2004, 22(2): 137. [17] ZHANG Wei-jie(张惟杰). Biochemical Research Technique On Carbohydrate Complexes(糖复合物生化研究技术). Hangzhou: Zhejiang University Press(杭州：浙江大学出版社), 1999. 193. [18] Wilkinson K J, Balnois E, Leppard G G, et al. Colloids Surf., A. 1999, 155: 287. [19] Gunning A P, Kirby V J, Morris V J, et al. Polym. Bull., 1995, 34(5-6): 615. [20] Camesano T A, Wilkinson K J. Biomacromolecules, 2001, 2(4): 1184. [21] Balnois E, Stoll S, Wilkinson K J, et al. Macromolecules, 2000, 33(20): 7440. [22] Decho A W. Carbohydr. Res., 1999, 315(3): 330. [23] Jin Y, Zhang H, Yin Y, et al. Carbohydr. Res., 2006, 341(1): 90. [24] Balnois E, Wilkinson K J. Colloids Surf., A., 2002, 207(1): 229. [25] Spagnoli C, Korniakov A, Ulman A, et al. Carbohydr. Res., 2005, 340(5): 929. [26] Morris V J, Mackie A R, Wilde P J, et al. Lebensmittel Wissenschaft and Technologie-Food Science Technology, 2001, 34(1): 3.
