Determination of Glycyrrhizic Acid in Glycyrrhiza Uralensis Fisch by Fiber Optic Near Infrared Spectroscopy
WANG Li1, 2,HE Ying1, 2,QIU Zhao-chai2, WANG Xiao-ru2, LEE Frank S C2
1. Department of Chemistry and the Key Laboratory of Analytical Science of MOE, Xiamen University, Xiamen 361005, China 2. State Oceanic Administration, First Institute of Oceanography, Qingdao 266061, China
Abstract：The objective of this study was to develop a rapid, non-destructive and real-time method for the determination of glycyrrhizic acid in glycyrrhiza uralensis fisch. Near infrared spectra (NIR) in the range of 10 000-4 000 cm-1 were recorded for the glycyrrhiza uralensis fisch containing glycyrrhizic acid in the content of 0.94%-3.06%. Calibration models were established using the PLS (partial least squares) and PCR (principle component regression) algorithm. Different spectra pretreatments methods were compared. The study showed that PLS model gave better results than PCR with the correlation coefficient 0.958, SEC 0.179(standard deviation of the calibration sets) and SEP 0.197 (standard deviation of the prediction sets). Results indicate that fiber optic NIR method can be used to on-line control the valid component in Chinese herbs.
Key words：Glycyrrhizic acid;Fiber optic near infrared spectroscopy;PLS
王 丽1, 2，何 鹰1, 2，邱招钗1，王小如2，黎先春2 . 光纤近红外光谱法在中草药分析中的应用——甘草中甘草酸含量的测定[J]. 光谱学与光谱分析, 2005, 25(09): 1397-1399.
WANG Li1, 2,HE Ying1, 2,QIU Zhao-chai2, WANG Xiao-ru2, LEE Frank S C2 . Determination of Glycyrrhizic Acid in Glycyrrhiza Uralensis Fisch by Fiber Optic Near Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1397-1399.
