1. State Key Laboratory of Physical Chemistry of Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
2. Zhejiang Provincial Hospital of TCM, Hangzhou 310006, China
3. Shenzhen Research Institute of Xiamen University, Shenzhen 518000, China
Abstract:The components of saliva are closely related to the corresponding components in blood. Using saliva instead of blood for detection can greatly reduce analysis time, limitation and potential safety hazard, thus it is of great importance to clinical research and control of drugs. In this work, combined with a portable Raman spectrometer, quantitative detection of pioglitazone hydrochloride (an oral hypoglycemic drug) in saliva has been realized by surface-enhanced Raman spectroscopy (SERS). With this method, the characteristic Raman peaks for pioglitazone hydrochloride can be easily observed. Furthermore, the SERS method can be used to quantitatively detect the pioglitazone hydrochloride in saliva. The concentration of pioglitazone hydrochloride is linearly correlated to its Raman intensity with a coefficient of determination (R2) of 0.992 3, and the minimum detectable concentration can be as low as 10 μg·L-1.
[1] Kaufman E, Lamster I B. Critical Reviews in Oral Biology & Medicine, 2002, 13(2): 197.
[2] Pfaffe T, Cooper-White J, Beyerlein P, et al. Clinical Chemistry, 2011, 57(5): 675.
[3] CUI Zi-jian, YAO Yu-lu, WANG Yan, et al(崔子健, 姚雨露, 王 艳,等). The Journal of Light Scattering(光散射学报), 2012, 24(1):40.
[4] LI Min-hua, TANG Jian(李敏华, 唐 健). Chinese Journal of Tissue Engineering Research(中国组织工程研究), 2012, 16(11):2071.
[5] HU Su-juan, WEN Lei-lei, CUI Yong(胡素娟, 闻雷雷, 崔 勇). Practical Journal of Clinical Medicine(实用医院临床杂志), 2013, 10(1): 39.
[6] CHEN Yue, ZHU Jun, ZHANG Yun-feng, et al(陈 跃, 朱 军, 张云峰, 等). Chinese Journal of Forensic Medicine(中国法医学杂志), 2016, 31(1): 9.
[7] ZOU Da-jin, WANG Qi-jin(邹大进, 王奇金). Chinese Journal of New Drugs(中国新药杂志), 2001, 10(6):469.
[8] LIAN Jiang-ping, ZHANG Peng, LI Xiao-chuang, et al(廉江平, 张 鹏, 李小川, 等). China Pharmacy(中国药房), 2005, 16(9):687.
[9] FANG Yu, ZHANG Hong, LI Ying(方 昱, 张 虹, 李 英). Chinese Journal of Hospital Pharmacy(中国医院药学杂志), 2008, 28(15):1262.
[10] Fleischmann M, Hendra P J, McQuillan A J. Chemical Physics Letters, 1974, 26: 163.
[11] Moskovits M. Reviews of Modern Physics, 1985, 57: 783.
[12] Jeanmaire D L, Van Duyne R P. Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1977, 84: 1.
[13] Tian Z Q, Ren B. Encyclopedia of Electrochemistry. New York: Wiley-VCH Verlag GmbH & Co. KGaA, 2007.
[14] WANG Lin, WANG Xue, TIAN Jing-miao, et al(王 琳, 王 雪, 田静秒, 等), Science and Technology of Food Industry(食品工业科技), 2016, 37(13):295.