Study on the Intermolecular Interaction of Icarrin with Human Serum Albumin Based on Fluorescence Spectrometry and Physical Modeling
ZHAO Xiao-xue1, GUO Ming1, 2*, WANG Yan1
1. School of Engineering, Zhejiang Agricultural & Forestry University, Lin’an 311300, China
2. School of Science, Zhejiang Agricultural & Forestry University, Lin’an 311300, China
Abstract：The intermolecular interaction between Icarrin (ICA) and Human Serum Albumin (HSA) was investigated by spectral method and building molecular model to explore ICA-HSA mechanism of interaction. And the intermolecular interaction model of ICA-HSA was described by using a variety of theoretical equations to compare and discuss their usability. The results showed that the fluorescence intensity of HSA in the fluorescence spectra showed regular decrease, indicating that HSA did interact with ICA. The synchronous fluorescence spectrum showed that micro-conformation of HSA changed due to the intermolecular interaction between ICA and HSA. The intermolecular interactions of ICA-HSA were described using Stern-Volmer equation, Lineweaver-Burk equation, double logarithmic regression equation, Scatchard series equation, Lehrer-Fasman equation and Tachiya model respectively. The results showed that the theoretical model was different and the results were different from ICA-HSA. There are two quantitative kinds of trends based on the differences between hypothesis and experimental conditions. In this paper, physical model was built to assist the intermolecular interaction mode of ICA-HSA.The optimal mode of ICA-HSA intermolecular interaction was the double logarithmic equation. Meanwhile, the ICA-HSA intermolecular interaction was analyzed by physical modeling, indicating that the ICA tend to occurr in active site Sudlow’s sites I and ICA-HSA system mainly has van der Waals force, hydrophobic interaction and hydrogen bonding. These results obtained in this paper serves to provide a reference for the comprehensive analysis of ICA-HSA intermolecular interaction, as well as the theoretical description of the intermolecular interactions between small molecules and biomacromolecules.
Key words：Icarrin; Human serum albumin; Spectroscopy; Docking; Intermolecular interaction
赵晓雪，郭 明，王 燕. 荧光光谱法及物理模建分析小檗淫羊藿甙与清蛋白的分子间作用[J]. 光谱学与光谱分析, 2018, 38(11): 3420-3427.
ZHAO Xiao-xue, GUO Ming, WANG Yan. Study on the Intermolecular Interaction of Icarrin with Human Serum Albumin Based on Fluorescence Spectrometry and Physical Modeling. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3420-3427.
[1] WANG Jia-guo, JING Xi-ping(王稼国，荆西平). University Chemistry(大学化学), 2012, 27(4): 83.
[2] HE Xiao-song, XI Bei-dou, WEI Zi-min, et al(何小松, 席北斗, 魏自民, 等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(10): 1417.
[3] Ahmed B, Surat H, Robert C, et al. Plos One, 2012, 7(5): e36723.
[4] GUO Qing-lian, HE Huan, PAN Ling-li, et al(郭清莲, 何 欢, 潘凌立, 等). Acta Physico-Chimica Sinica(物理化学学报), 2016, 32(6): 1383.
[5] Shi J H, Wang J, Zhu Y Y, et al. Luminescence the Journal of Biological & Chemical Luminescence, 2014, 29(5): 522.
[6] Fang F, Pan D Q, Qiu M J, et al. Luminescence the Journal of Biological & Chemical Luminescence, 2016, 31(6): 1242.
[7] Fotiou M, Michaelidou A M, Athanasiadis, et al. Adv. Mater. Sci. Eng., 2015, 50: 358.
[8] ZHANG Bing-wei, XIA Wen-shui, DING Li, et al(张冰卫, 夏文水, 丁 黎, 等). Progress in Pharmaceutical Sciences(药学进展), 2011, 7: 296.
[9] Tabassum S, Al-Asbahy W M, Arjmand F, et al. J. Photochem. Photobiol. B, 2012, 114: 132.
[10] Qin Y, Zhang Y X, Yan S L, et al. Spectrochimica Acta Part A, 2010, 75: 1506.
[11] Bolli A, Marino M, Rimbach G, et al. Biochemical and Biophysical Research Communications, 2010, 398(3): 444.
[12] DONG Cheng-yu, XU Jia, ZHOU Shan-shan, et al(董澄宇, 徐 佳, 周珊珊, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2017, 37(1): 327.
[13] Tian F F, Jiang F L, Han X L, et al. J. Phys. Chem. B, 2010, 114: 14842.
[14] Poureshghi F, Ghandforoushan P, Safarnejad A, et al. Journal of Photochemistry & Photobiology B Biology, 2016, 166: 187.
[15] He L L, Wang Z X, Wang Y X, et al. Colloids & Surfaces B Biointerfaces, 2016, 145: 820.
