1. Gansu Center for Disease Control and Prevention，Lanzhou 730000，China 2. Pharmic College of Harbin University of Commerce, Harbin 150076，China 3. Department of Chemistry, Tsinghua University, Beijing 100084，China
Abstract：Illicium vatum Hook. f. and its counterfeit Illicium lanceolatum A.C. Smith can be discriminated and identified by using multi-steps infrared maro-fingerprint method. The method combines three steps: general infrared spectroscopy (FTIR), secondary derivative spectroscopy and two dimensional correlation infrared spectroscopy (2D-IR). The resolution is getting higher and higher and the differences of spectra are magnified farther. The holistic shape of peaks is similar in the FTIR spectra of Illicium vatum Hook. f. and Illicium lanceolatum A.C. Smith, but the spectra of llicium vatum Hook. f. show only one characteristic peak at 3 392 cm-1, while the spectra of Illicium lanceolatum A.C. Smith show two characteristic peaks at 3 482 and 3 387 cm-1, respectively. Observing their secondary derivative spectra, in the range of 850-1 180 cm-1 the strongest peak of llicium vatum Hook. f. is at 1 015 cm-1 and the intensity of other strong peaks is similar. However, the strongest peak of Illicium lanceolatum A.C. Smith is at 1 070 cm-1. In 1 180-1 500 cm-1, the intensity of peaks at 1 469，1 454 and 1 442 cm-1 of llicium vatum Hook. f. is stronger than that of peaks at 1 292，1 276，1 266 cm-1. The status of Illicium lanceolatum A. C. Smith is just opposite. The distinction of 2D-IR correlation spectra is more obvious. In 1 165-1 500 cm-1, the stronger automatic peaks of llicium vatum Hook. f. present at 1 153 and 1 000 cm-1, respectively, but those of Illicium lanceolatum A. C. Smith present at 911 and 878 cm-1, respectively. In 1 165-1 500 cm-1, llicium vatum Hook. f. has two automatic peaks while Illicium lanceolatum A. C. Smith has five automatic peaks. The multi-steps IR macro-fingerprint method is rapid and effective.
Key words：Illicium vatum Hook. f. and Illicium lanceolatum A. C. Smith;FTIR;Secondary derivative spectra;2D-IR correlation spectra
周晶1,3, 孙建云1,徐胜艳2,周群3,孙素琴3* . 八角茴香与其伪品莽草的红外光谱三级鉴定研究[J]. 光谱学与光谱分析, 2008, 28(12): 2864-2867.
ZHOU Jing1,3,SUN Jian-yun1,XU Sheng-yan2,ZHOU Qun3,SUN Su-qin3*. Study on the Identification of Illicium Vatum Hook. f. and Illicium Lanceolatum A. C. Smith by Multi-Ateps Infrared Macro-Fingerprint Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(12): 2864-2867.
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