1. 吉林农业科技学院农学院，吉林 吉林 132101
2. Institute of Agricalture, The University of Tennessee, Knoxville, TN 37996, USA
Structure Characteristics of Mineral-Microbial Residues Formed by Microbial Utilization of Lignin Based on the Participations of Different Clay Mineral Types
WANG Shuai1,2, XU Jun-ping1, CHEN Dian-yuan1*, JIANG Shuai1, LI Xing-ji1, SHENG Bing-han1, Schaeffer Sean2
1. College of Agricultural Sciences, Jilin Agricultural Science and Technology University, Jilin 132101, China
2. Institute of Agricalture, The University of Tennessee, Knoxville, TN 37996, USA
Abstract：The catalytic action of clay minerals have an important contribution to the formation of humic substance (HS) from the lignin. In order to elucidate the relationship among the microorganisms, lignin and clay mineral effectively and reveal the structural characteristics of mineral-microbial residues, the method of liquid shake flask culture was adopted in this article, the lignin serving as the sole C source, through the addition of kaolinite or montmorillonite to start the liquid culture of 110 days after inoculating the multiple strains, and then the mineral-microbial residues were dynamically collected and their characteristics were studied by FT-IR and SEM techniques. The results were as follows: The kaolinite particles were mostly formed from the crimp of tube-like material edges. After its participation in the formation of microbial utilization of lignin, much more structures from the fine particles of mineral-microbial residues were further aggregated and they were more integrated, in the process the structures like short tubular were increased, but the overall state still maintained the structural characteristics of hydro-kaolinite. Under the initial culture with a rich variety of nutritive elements, the kaolinite could promote the microbial reproduction, which could make a large number of microorganisms gathered on the kaolinite surface and the Si—O and Si—O—Al bonds were masked. During the process, the proportion of aliphatic C structure of mineral-microbial residues were increased; The H bonds could be formed from the conjunction of multiple O-containing functional groups of high-molecular polysaccharides and the hydration shell of kaolinite at the multiple sites. The formation of H bonds had significant effect to stabilize the lignin and its degradation products from the kaolinite. With the culture, the proportion of aromatic C structure and the polysaccharides content were gradually increased, and then the microbial residues masked on the surface of kaolinite were utilized again by the multiple strains with active ability, which could make its Si—O—Al bond reappeared; Montmorillonite was mostly composed of round particles, and the dissolution was caused by the microbial inoculation on its surface, which could make the granular structures broken and produce much more fragmented structures. Compared with 10 days, the polysaccharides of mineral-microbial residues obtained from the culture of 30 days were increased, which could make the absorption peak at 1 034~1 038 cm-1 assigned as the Si—O—Si and Si—O bonds overlayed and strengthened, and then the intensity of absorption peak at 1 034~1 038 cm-1 was weakened due to the association of polysaccharides with hydroxyl of montmorillonite surface, and simultaneously the intermolecular H bonding occurred, which was the main mechanism for the interaction of montmorillonite, microorganisms and lignin and their formation of mineral-microbial residues. The ability to stabilize organic C from the kaolinite was more than montmorillonite, which was easier to promote the formation of HS precursor substances.
王 帅，徐俊平，陈殿元，姜 帅，李兴吉，盛炳翰，Schaeffer Sean. 粘土矿物参与微生物利用木质素形成矿物-菌体残留物的结构特征研究[J]. 光谱学与光谱分析, 2018, 38(09): 2903-2909.
WANG Shuai, XU Jun-ping, CHEN Dian-yuan, JIANG Shuai, LI Xing-ji, SHENG Bing-han, Schaeffer Sean. Structure Characteristics of Mineral-Microbial Residues Formed by Microbial Utilization of Lignin Based on the Participations of Different Clay Mineral Types. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2903-2909.
[1] Tuomela M, Oivanenl P, Hatakka A. Soil Biology & Biochemistry, 2002, 34: 1613.
[2] Smidt E, Meissl K, Schmutzer M, et al. Industrial Crops & Products, 2008, 27(2): 196.
[3] najdr J, Steffen K T, Hofrichter M, et al. Soil Biology & Biochemistry, 2010, 42(9): 1541.
[4] Mapelli F, Marasco R, Balloi A, et al. Journal of Biotechnology, 2012, 157(4): 473.
[5] Liang C, Cheng G, Wixon L C, et al. Biogeochemistry, 2011, 106(3): 303.
[6] Miltner A, Bombach P, Schmidt-Brücken B, et al. Biogeochemistry, 2012, 111(1-3): 41.
[7] Schmidt M W, Torn M S, Abiven S, et al. Nature, 2011, 478(7367): 49.
[8] Wattel-koekkoek E J W, Buurman P, Vander J P, et al. European Journal of Soil Science, 2003, 54: 269.
[9] Kaiser K, Guggenberger G, Derenne S, et al. Organic Geochemistry, 2000, 31: 711.
[10] Six J, Conant R T, Paul E A, et al. Plant and Soil, 2002, 241: 155.
[11] Filip Z, Haider K. Soil Biology & Biochemistry, 1972, 4(2): 147.
[12] BAO Zhen-hong, JIANG Wei-hui, MIAO Li-feng, et al(包镇红，江伟辉，苗立锋，等). Journal of Ceramics(陶瓷学报)，2014，35(1)：53.
[13] HUANG Ming, LI Shao-feng, LU Xiu-guo, et al(黄 明，李绍峰，鲁秀国，等). Chinese Journal of Environmental Engineering(环境工程学报)，2016，10(11)：6439.
[14] Chaerun S K, Tazaki K. Clay Minerals, 2005, 40: 481.
[15] XI Jian-hong, HE Meng-chang, LIN Chun-ye, et al(席建红，何孟常，林春野，等). Environmental Chemistry(环境化学)，2009，28(1)：54.
[16] HAO Qing-li, YANG Xu-jie, WANG Ying(郝青丽，杨绪杰，王 瑛). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2000，20(3)：302.
[17] Kepelová A, Reich T, Sachs S, et al. Journal of Colloid and Interface Science, 2008, 319(1): 40.
[18] He X S, Xi B D, Jiang Y H, et al. Microchemical Journal, 2013, 106: 160.
[19] JIA Chun-yun, LI Pei-jun, WEI De-zhou, et al(贾春云，李培军，魏德洲，等). Microbiology China(微生物学通报)，2010，37(4)：607.
[20] ZUO Xiao-chao, LIU Qin-pu, JI Jing-chao, et al(左小超，刘钦甫，姬景超，等). Journal of the Chinese Ceramic Society(硅酸盐学报)，2015，43(9)：1294.
[21] YIN Yong-yuan, GUO Xue-tao, YANG Chen, et al(尹永远，郭学涛，杨 琛，等). Environmental Chemistry(环境化学)，2017，36(3)：572.
[22] Zhang X L, Niu H Y, Li W H, et al. Chemical Communications, 2011, 47(15) : 4454.
[23] Oren A. International Journal of Systematic Bacteriology, 1983, 33: 381.
[24] Rong X M, Huang Q Y, He X M, et al. Colloida and Surfaces B: Biointerfaces, 2008, 64(1): 49.
[25] Zhou Y, Chen H, Yao J, et al. Applied Clay Science, 2010, 50(4): 533.
[26] HUA Li, JIN Su-su, LUO Jing-jing(花 莉，金素素，洛晶晶). Ecology and Environmental Sciences(生态环境学报)，2012，21(11)：1795.
[27] Subramanian S, Santhiya D, Natarajan K A. International Journal of Mineral Processing, 2002, 72(10): 175.
