Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy
Vikas Sarsar1, Manjit K. Selwal1, Krishan K. Selwal1, 2*
1. Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, India
2. Department of Animal Sciences, The Ohio State University, Columbus (Ohio), USA
Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy
Vikas Sarsar1, Manjit K. Selwal1, Krishan K. Selwal1, 2*
1. Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, India
2. Department of Animal Sciences, The Ohio State University, Columbus (Ohio), USA
摘要： The aim of this study to focused on bioinspired synthesis of silver nanoparticles (AgNPs) as a viable alternative to eradicate the existing physicochemical processes. In this context, the bioinspired AgNPs were synthesized using leaf extract of M. indica. Optimization of the experimental conditions for the rapid and high yield of AgNPs in minimum investment of time and expense have been carried out along with their antibacterial efficacy evaluated. For this reason, the variation of parameters like the concentration of the silver precursors, reducing agent, time, pH, and temperature of synthesis were realized. Synthesized AgNPs were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) techniques. UV-Visible spectra gave surface plasmon resonance (SPR) at 440 nm for AgNPs. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques were further confirmed the synthesis and crystalline nature of AgNPs respectively. Transmission electron microscopy (TEM) observed spherical shapes of synthesized AgNPs within range 5～20 nm. The results of the current study indicate that optimization process play a pivotal role in the AgNPs synthesis and biogenic synthesized AgNPs might be used against bacterial pathogens.
关键词：AgNPs; Antibacterial efficacy; FTIR; TEM; XRD
Abstract：The aim of this study to focused on bioinspired synthesis of silver nanoparticles (AgNPs) as a viable alternative to eradicate the existing physicochemical processes. In this context, the bioinspired AgNPs were synthesized using leaf extract of M. indica. Optimization of the experimental conditions for the rapid and high yield of AgNPs in minimum investment of time and expense have been carried out along with their antibacterial efficacy evaluated. For this reason, the variation of parameters like the concentration of the silver precursors, reducing agent, time, pH, and temperature of synthesis were realized. Synthesized AgNPs were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) techniques. UV-Visible spectra gave surface plasmon resonance (SPR) at 440 nm for AgNPs. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques were further confirmed the synthesis and crystalline nature of AgNPs respectively. Transmission electron microscopy (TEM) observed spherical shapes of synthesized AgNPs within range 5～20 nm. The results of the current study indicate that optimization process play a pivotal role in the AgNPs synthesis and biogenic synthesized AgNPs might be used against bacterial pathogens.
通讯作者:
Krishan K. Selwal
E-mail: krishan.kselwal@gmail.com
作者简介: sarsarvikas@gmail.com
引用本文:
Vikas Sarsar, Manjit K. Selwal, Krishan K. Selwal. Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy[J]. 光谱学与光谱分析, 2018, 38(08): 2624-2629.
Vikas Sarsar, Manjit K. Selwal, Krishan K. Selwal. Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2624-2629.
[1] Amendola V, Polizzi S, Meneghetti M. Langmuir，2007，23: 6766.
[2] Cao Y W, Jin R, Mirkin C A. J. Am. Chem. SOC, 2001, 123: 7961.
[3] Caruthers S D, Wickline S A, Lanza G M. Curr. Opin. Biotechnol., 2007, 18: 26.
[4] Gracias D H, Tien J, Breen T, et al. Science，2002，289: 1170.
[5] Hullmann A. Scientomet，2009，70: 739.
[6] Kamat P V. J. Phy. Chem. B, 2002, 106: 7729.
[7] Krishnaraj C, Jagan E G, Rajasekar S, et al. Colloids Surf B Biointerfaces，2010，76: 50.
[8] Maier S A, Brongersma M L, Kik P G, et al. Advanced Materials，2001，19: 1501.
[9] Matejka P, Vlckova B, Vohlidal J, et al. J. Phys. Chem., 1992, 96: 1361.
[10] Mccuen R H. JAWRA Journal of the America Association, 2009, 45: 1063.
[11] Mirkin C A, Letsinger R L, Mucic R C, et al. Nature, 1996, 382: 607.
[12] Prathna T C, Chandrasekaran N, Raichur A M, et al. Colloids Surf B Biointerfaces，2011，82: 152.
[13] Reddy A S, Chen C Y, Chen C C, et al. J. Nanosci. Nanotechnol., 2010，10: 6567.
[14] Rodrigues A, Ping L, Tasic L, et al. App. Microbio. Biotech.，2013，doi: 10.1007/s00253-012-4209-7.
[15] Sandhu A. Nat. Nanotech., 2008，2008: 120.
[16] Sarsar V, Selwal K K, Selwal M K. J. Saudi. Chem. Soc., http://dx.doi.org/10.1016/j.jscs. 2014.07.001.
[17] Sarsar V, Selwal K K, Selwal M K. Afr. J. Biotech., 2014，13(4): 546.
[18] Sarsar V, Selwal K K, Selwal M K. Pharmanest，2014，5(1): 1769.
[19] Shahverdi A R, Fakhimi A, Shahverdi H R, et al. Nanomed. Nanotechnol. Biol. Med., 2007, 3: 168.
[20] Shan G, Surampalli R Y, Tyagi R D, et al. Front. Environ. Sci. Engin. China, 2009, 3: 249.
[21] Shankar S S, Rai A, Ahmad A, et al. J. Colloid. Interface Sci., 2004，275: 496.
[22] Tolaymat T M, El Badawy A M, Genaidy A, et al. Sci. Total Environ., 2010, 408: 999.
[23] Tripathy A, Raichur A M, Chandrasekaran N, et al. J. Nanopart. Res., 2010, 12: 237.
[24] Vaidyanathan R, Gopalram S, Kalishwaralal K, et al. Colloids Surf B，2010，75(1): 335.
[25] Veerasamy R, Zi T, Gunasagaran S, et al. J. Saudi. Chem. Soc., 2011, 15(2): 113.
[26] Venkatesan B, Subramanian V, Tumala A, et al. Asian Pac. J. Trop Med., 2014，7(Suppl 1): S294.
[27] Zach M, Hagglund C, Chakarov D, et al. Curr. Opi. Sol. Sta. Mat. Sci., 2006, 10: 132.
